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講者：李思禹教授

演講題目：Mixotrophic Escherichia coli for bio-based chemical productions

演講時間：5/22（五）15:30~17:20

演講地點：普通教室 101

主持人：謝之真教授

ABSTRACT

Carbon dioxide is a ubiquitous molecule produced by most industrial activities. With the evident CO₂ accumulation in the atmospheric layer over the past few decades, more and more research attentions have been paid to reduce the CO₂ emission or to recover emitted CO₂. Fermentation process is highly associated with the CO₂ emission. For example, 1 mole of bioethanol being produced is equivalent to 1 mole-CO₂ emitted, considering the microbial fermentation process as a whole. Meanwhile, 1 mole of biobutanol is roughly 4-mole CO₂ equivalent using Clostridium acetobutylicum. Although the concept of biofuels is to reach carbon neutral by using biomass as the feedstock, it is desirable to take one step further to reduce the CO₂ emission during fermentation. One potential way is to metabolically turn industrial bacteria from heterotrophs to mixotrophs, where mixtrophs can use both carbohydrates and CO₂ as carbon sources. In this manner, the CO₂ emission can be recycled by bacteria itself while those recycled CO₂ can be used to make more fermentation products. In this study, the feasibility of mixotrophic Escherichia coli will be discussed. The mixotrophic Escherichia coli is constructed based on the Calvin cycle, where two key genes encoding  ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and phosphoribulokinase (PrkA) was cloned and overexpressed in E. coli. By further appropriate metabolic engineering on the central metabolism, Rubisco-based engineered E. coli can recycle CO₂ during the metabolism of not only pentoses but also hexoses.

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