台大化工系網站英文版

講者：李思禹教授

演講題目：Mixotrophic
Escherichia coli for bio-based chemical productions

演講時間：5/22（五）15:30~17:20

演講地點：普通教室 101

主持人：謝之真教授

ABSTRACT

Carbon
dioxide is a ubiquitous molecule produced by most industrial activities. With
the evident CO₂ accumulation in the atmospheric layer over the past
few decades, more and more research attentions have been paid to reduce the CO₂
emission or to recover emitted CO₂. Fermentation process is highly
associated with the CO₂ emission. For example, 1 mole of bioethanol
being produced is equivalent to 1 mole-CO₂ emitted, considering the
microbial fermentation process as a whole. Meanwhile, 1 mole of biobutanol is
roughly 4-mole CO₂ equivalent using Clostridium acetobutylicum. Although the concept of biofuels is to
reach carbon neutral by using biomass as the feedstock, it is desirable to take
one step further to reduce the CO₂ emission during fermentation. One
potential way is to metabolically turn industrial bacteria from heterotrophs to
mixotrophs, where mixtrophs can use both carbohydrates and CO₂ as
carbon sources. In this manner, the CO₂ emission can be recycled by
bacteria itself while those recycled CO₂ can be used to make more
fermentation products. In this study, the feasibility of mixotrophic Escherichia coli will be discussed. The
mixotrophic Escherichia coli is
constructed based on the Calvin cycle, where two key genes encoding  ribulose-1,5-bisphosphate
carboxylase/oxygenase (Rubisco) and phosphoribulokinase (PrkA) was cloned and
overexpressed in E. coli. By further
appropriate metabolic engineering on the central metabolism, Rubisco-based
engineered E. coli can recycle CO₂
during the metabolism of not only pentoses but also hexoses.

關於本次演講資訊請參考附件檔案，謝謝。