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講者：蘭宜錚教授
演講題目：Towards
sustainability through metabolic engineering of microbial cell factories
演講時間：3/13(五) 3:30~5:00
pm
演講地點：普通教室101教室
主持人：謝之真教授
Towards sustainability through
metabolic engineering of
microbial cell factories
Ethan
I Lan
Department of Biological Science
and Technology, National Chiao Tung University
Increasing concerns over global climate change
and eventual depletion of fossil fuel resources are two of the most significant
challenges of this century. Production of biofuel and biochemical is a
promising approach to solving both of these challenges. However, natural
microorganisms have limited ability to produce the diverse fossil fuel derived
products needed in modern society. To address this limitation, metabolic
engineering of microbial cell factories can develop better whole cell
bio-catalysts to convert renewable biological feedstock into replacements for
petrochemicals and fuels. Among the various bioresources utilization
strategies, the direct conversion of CO2 to useable chemicals by
microalgae and cyanobacteria is an attractive approach because it bypasses the
need of plant growth and the difficulty of plant biomass recalcitrance.
1-Butanol is a potential gasoline surrogate and
a chemical feedstock used in many industries. We
engineered a model cyanobacterium Synechococcus
elongatus PCC 7942 for the production of 1-butanol. The natural Clostridium 1-butanol production pathway
is not compatible with cyanobacteria for functional expression. As a result,
direct photosynthetic production of 1-butanol has been limited. To redesign the
pathway for functional expression in cyanobacteria, we engineered a synthetic
driving force into the pathway and solved the problem of enzyme oxygen
sensitivity by recruiting enzymes from 1,2-propanediol degradation pathway. To
further increase the chemical diversity that we can produce using microbes, we
extend the pathway for 1-butanol biosynthesis to produce 1-hexanol and
2-pentanone using Escherichia coli.
關於本次演講資訊請參考附件檔案，謝謝。
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